On culture artefacts in coccolith morphology

Langer, Gerald and Oetjen, Kerstin and Brenneis, Tina (2013) On culture artefacts in coccolith morphology. Helgoland Marine Research, 67 (2). pp. 359-369. ISSN 1438-387X, ESSN: 1438-3888 DOI 10.1007/s10152-012-0328-x

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Abstract

Coccolith malformations occur more frequently in cultured specimens than in specimens from natural samples, a phenomenon commonly termed ‘culture artefacts’. The causes of culture artefacts are unknown. Here, we tested the effect of culture flask shape, mixing, and cell density on the morphology of Emiliania huxleyi coccoliths. While there was no effect of different culture flask types typically used in coccolithophore culturing, continuous mixing reduced the percentage of malformations by ca. 11 % in exponential-phase cells (cell density ca. 80 × 103 cells per ml) and ca. 17 % in stationary-phase cells (cell density ca. 2 × 106 cells per ml). Stationary-phase cells displayed 19 % more malformations than mid-exponential-phase cells when not mixed at all and 20 % more malformations when continuously mixed. It is concluded that the lack of mixing and unnaturally high cell densities, typical for coccolithophore stock cultures, are partly responsible for culture artefacts.

Item Type:	Article
Uncontrolled Keywords:	2013AREP, IA66,
Subjects:	04 - Palaeobiology
Divisions:	04 - Palaeobiology
Journal or Publication Title:	Helgoland Marine Research
Volume:	67
Page Range:	pp. 359-369
Identification Number:	10.1007/s10152-012-0328-x
Depositing User:	Sarah Humbert
Date Deposited:	07 Jul 2013 00:41
Last Modified:	08 Aug 2013 12:14
URI:	http://eprints.esc.cam.ac.uk/id/eprint/2817

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